Before the development of antibiotics, even a small cut could have fatal results if a bacterial infection took hold. Today, the world faces a the return of untreatable diseases due to antibiotic resistance.
Prof Raymond J. Turner at the University of Calgary is working to solve the problem of bacterial resistance to these medicines through revisiting and developing metal-containing antimicrobials.
Read the original paper here: https://doi.org/10.3390/antibiotics9120853
Read more in Research Features
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Hello and welcome to Research Pod. Thank you for listening and joining us today. In this episode we will be looking at the research of Professor Raymond J. Turner into antimicrobial metals.
Antibiotics are one of the key factors facilitating the increase of life expectancies by nearly 30 years in the US. Before the development of antibiotics, even a seemingly innocuous cut could have fatal results when a bacterial infection took hold. However, with the high use of these wonder drugs, bacterial resistance has become an increasing problem, and one that Professor Raymond J. Turner at the University of Calgary is working to solve, through revisiting and developing metal-containing antimicrobials.
Antibiotic and antimicrobial resistance has the potential to become one of the biggest threats to global health and food security as we find ourselves entering an antimicrobial resistance (AMR) era. Already, many microbes are developing partial or have developed complete antimicrobial resistance leading to the rise of the so-called ‘superbugs’ – resistant strains of microbes that are invulnerable to some or all of our treatment options.
The consequences of antimicrobial resistance can be severe. Even very minor surgeries would become almost intolerably risky as any post-operative infection could prove fatal. Life expectancies would decrease dramatically as there would be no way of controlling the development of initially minor infections. There is already some evidence to suggest that antibiotic resistance is having a negative impact on life expectancy and diseases such as urinary tract infections, gonorrhoea and tuberculous are already becoming increasingly difficult to treat with available therapies.
Why has the problem become so bad? One theory is that the over prescription and misuse of antibiotics, particularly in the agriculture industry as a prophylaxis and in mismanagement of infections has helped bacteria develop resistance to common therapies. This is because unless the bacterial population is completely annihilated by the treatment, the bacteria that remain tend to be those with the highest levels of tolerance against the drug. These then go on to multiply, passing on the genetic information that gave them a higher level of resistance. If this cycle continues, the problem worsens until treatments become completely ineffective.
One way of circumventing resistance issues is to try treating with different classes of antimicrobials, in the hope that the bacteria will not have developed resistance to all of them. Unfortunately, this strategy is also becoming less effective as there have been no new major classes of antibiotics developed in the last 20 years, so trialling different antibiotic classes as part of treatment has also led to the development of greater resistance.
Professor Raymond J. Turner at the University of Calgary has been investigating how metal ions might help us in the fight against bacteria. Many metal species, including those of copper and silver, have known antimicrobial properties and Professor Turner has been investigating which metals might be the best at destroying biofilms and other bacterial growth forms.
The amazing antimicrobial properties of metals have been known since 4 BC, when Hippocrates, often considered the father of modern medicine, was using ointments of metals as part of treatment. The ability of metals to kill bacterial species is why some bandages and sports equipment have silver infused into their fibers. These textiles can help keep wounds sterile or kill the skin bacteria feeding on sweat to prevent odours.
Professor Turner and his team have been systematically investigating which metals – silver, gold, copper, titanium, gallium, nickel, aluminium, tellurium, selenium, zinc, and others – would offer the best antimicrobial activity against different strains of bacteria. They wanted to discern which metals would be most efficient at eradicating already established biofilms of the bacteria and which would prevent any future growth in the region.
Biofilms are the consortium of bacteria that form and stick to surfaces as the bacterial community grows. They are often slimy to the touch and can stick to surfaces and other areas, including the cells of wounds. The biofilm can help protect the bacteria as some films can even prevent treatments such as antibiotics entering the bacterial cells, stopping the treatment from working.
What Professor Turner’s group found was that, if he combined certain metal salts, some combinations proved to ber synergistic. That meant the bacterial killing or prevention activity of the combination was greater than the sum of the two individual components. The combination can also reduce or even prevent resistance from developing. Finding the right synergistic combination was dependent on the bacteria being targeted, with several metals, either as salts or nanomaterials, proving particularly promising.
Professor Turner and his group are keen to highlight the nuances of resistant bacteria. They point out that there are already silver-resistant bacteria, for example, arising because of misuse of silver within the textile industry. One might develop silver resistance as a result of silver used in garments, meaning in future, silver used in a bandage after a burn will offer little protection.
While we might have been reaping the antimicrobial benefits from metals since the time of Hippocrates, understanding why metals make good antimicrobials and how they work has been a long unanswered question.
There are a number of different ways metals can destroy bacteria. These include the generation of reactive oxygen species that can lead to cell death – a similar mechanism that is used in many cancer treatments to kill the unwanted cancerous cells. This can be via depletion of the antioxidant concentrations that would normally control the concentration of the reactive oxygen species to prevent cell damage.
Other routes include substitution of the natural metal in an enzyme, which can disrupt and inhibit its function. Some metals can also interfere with cell nutrient uptake so the cell essentially starves to death. More severe disruption can be caused by direct damage of the genetic material of the bacteria that can prevent reproduction, growth and function or damage of the cell membranes that act as both a safety barrier and a container for the cell contents.
Professor Turner has been investigating not just the more standard mechanisms of metal-bacterial interactions, but is also looking into the origins of synergistic effects of multiple metals on bacteria. While there are some concerns that the inherent cell toxicity of many metals will make them challenging for human use as antibiotic treatment, new abilities to specially manufacture nanomaterials doped with just the right amount of metal may help overcome some of these challenges.
Tackling antimicrobial resistance is an urgent challenge for scientists and finding new and novel families of compounds with antimicrobial activity is part of that. Professor Turner’s work shows the many possibilities of individual or combination metal-based therapies and how they can be used to fight even the most stubborn of pathogens.
That’s all for this episode – thanks for listening, and stay subscribed to Research Pod for more of the latest science. See you again soon.